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StriatechJournal Club

In Vivo Modeling of Immune-mediated Optic Neuropathies

Oliver W. Gramlich, Ph.D. - The University of Iowa
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Description

This presentation outlines the pathobiology and visual phenotype of immune-mediated optic neuropathies that include Multiple Sclerosis (MS), Neuromylitis optica spectrum disorders (NMOSD), and Myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD). All three disorders feature distinct clinicopathologic phenotypes. These phenotypes will be highlighted in relevant preclinical models of optic neuritis by utilizing highly translational functional and structural measurements of vision. The presentation features an introduction of relevant animal models, and explains pertinent experimental techniques ranging from behavioral assessment of visual acuity (VA) with the OptoDrum, to electrophysiology recordings via pattern electroretinography (pERG) and visual evoked potential (VEP) recordings, to structural imaging of the retina with optical coherence tomography (OCT).

Key Topics

  • Pathobiology and visual phenotype of immune mediated optic neuropathies
  • Novel models of NMOSD and MOGAD optic neuritis
  • Targeting cholesterol homeostasis mitigates vision loss in EAE

Learning Objectives

  • Introduction to the clinical relevance and pathobiology of optic neuritis.
  • Familiarize with novel, autoantibody-induced in vivo models of NMOSD and MOGAD optic neuritis.
  • Recap of the MOG-induced experimental autoimmune encephalomyelitis (EAE) optic neuritis model.
  • Summarize translational methods of visual function and structure (VA, pERG, VEP, OCT) for preclinical drug testing.

Background Reading

The changing landscape of optic neuritis: a narrative review. De Lott LB, Bennett JL, Costello F
J Neurol. 2022 Jan;269(1):111-124.
doi: 10.1007/s00415-020-10352-1.

Rodent Models of Optic Neuritis. Redler Y, Levy M.
Front Neurol. 2020 Nov 3;11:580951.doi: 10.3389/fneur.2020.580951.

Systemic Mesenchymal Stem Cell Treatment Mitigates Structural and Functional Retinal Ganglion Cell Degeneration in a Mouse Model of Multiple Sclerosis. Gramlich OW, Brown AJ, Godwin CR, Chimenti MS, Boland LK, Ankrum JA, Kardon RH.
Transl Vis Sci Technol. 2020 Jul 10;9(8):16.
doi: 10.1167/tvst.9.8.16.

About the speaker

Oliver W. Gramlich, Ph.D.

Oliver W. Gramlich, Ph.D.

Research Assistant Professor
The University of Iowa

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Oliver Gramlich studied Biology and Neurobiology at the University of Mainz followed by a Phd focusing on Experimental Ophthalmology. He then started a position at the University of Iowa where he is now working as Research Assistant Professor at the Department of Ophthalmology and Visual Sciences. Besides his research efforts on Multiple Sclerosis-related optic neuritis and glaucoma, he is currently establishing a model of Neuromyelitis optica in order to help establish reliable preclinical models that faithfully reproduce visual impairment as seen in patients.

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Journal Club: In Vivo Modeling of Immune-mediated Optic Neuropathies
Nov 09th, 2022

    Striatech Journal Club - The University of Iowa

    I already use optomotor measurementsI want to do optomotor measurements in the future

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