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March 11, 2025 • ✎ STRIA TECH

Nonlinear pathological trajectory of a high-myopia C57/BL6J mouse model induced by form deprivation

Topics:

Myopia

Myopia, Refractive Development and Eye Growth

Publication

Frontiers in Physiology (Oct 30, 2024) Nonlinear pathological trajectory of a high-myopia C57/BL6J mouse model induced by form deprivation

Wen Y, Li Y, Zhu L, Tang T, Yan H, Hu J, Wang K, Zhao M, Xu Q

DOI: 10.3389/fphys.2024.1442000 >>

This study established a high myopia model in C57BL/6J mice using monocular form deprivation myopia (FDM) and investigated its ocular structure changes over time. Researchers divided 3-week-old mice into FDM and control groups, patching the left eyes of the FDM group. After 12 weeks, deprived eyes showed significant changes, including longer axial length, higher refractive power, and thinner retina, choroid, and sclera. The retinal vascular area in covered eyes decreased, with an accelerated decline from weeks 8 to 12. The study successfully induced high myopia in mice, with axial length growing dramatically in the first 8 weeks and fundus pathology progressing faster thereafter. A Photorefractor was used to measure refraction in the mice’s eyes, providing accurate measurements of the induced myopic changes.

Introduction: To establish a high myopia model in C57BL/6J mice with monocular form deprivation myopia (FDM) and investigate its ocular structure pathological trajectory. Methods: Healthy 3-week-old C57BL/6J mice were divided into an FDM group (n = 36) and a control group (n = 24). The left eyes of the FDM group were patched, while the right eyes served as controls. Biometric parameters and fundus morphology were assessed at baseline and after 4, 8, and 12 weeks of form deprivation. Results: Significant differences were observed in the deprived eyes, including longer axial length, higher refractive power, deeper vitreous chambers, thinner retina, choroid, and sclera, and smaller scleral fibers' diameters under a transmission electron microscope. Retinal vascular area proportion in covered eyes decreased significantly (P < 0.05), with a decline rate of 11% from weeks 4 to 8 and a faster decline of 19% from weeks 8 to 12, while this proportion increased significantly in control eyes. Discussion: This study successfully induced a high myopia model in mice with long-term form deprivation. The axial length grew dramatically in FDM in the first 8 weeks, while the pathological progress of the fundus accelerated from weeks 8 to 12.